Abstract

Phalaris arundinacea L. (reed canary grass) is a widely occurring grass throughout the Northern Hemisphere. In North America, it is thought to consist of introduced agricultural forms from Europe as well as native populations.
During a survey of Phalaris arundinacea in western Canada, we discovered two distinct ribotypes in the sequences of the internal transcribed spacer (ITS) of the nuclear ribosomal DNA: one full length (ITS-long) and one with a seven base pair deletion (ITS-short). In addition, ITS-long plants have fixed heterozygosity indicating possible polyploidy. Phylogenetic analysis reveals that ITS-short is a unique ribotype that characterises an intraspecific clade. We designed an efficient PCR-based assay that allows sizing of a 238/245 base pair fragment in a capillary sequencer. This approach provides a novel marker that could be useful in future surveys of Phalaris arundinacea.

Addeddate

2024-09-09 16:11:52

Bhl_virtual_titleid

210877

Bhl_virtual_volume

v.11 (2023)

Call number

10_3897_BDJ_11_e101257

Call-number

10_3897_BDJ_11_e101257

Foldoutcount

0

Genre

article

Identifier

reporttwodistin11perc

Identifier-ark

ark:/13960/s29vgb2b4h1

Identifier-bib

10_3897_BDJ_11_e101257

Identifier-doi

10.3897/BDJ.11.e101257

Identifier-elocator

e101257

Ocr

tesseract 5.3.0-6-g76ae

Ocr_detected_lang

en

Ocr_detected_lang_conf

1.0000

Ocr_detected_script

Latin

Ocr_detected_script_conf

1.0000

Ocr_module_version

0.0.21

Ocr_parameters

-l eng

Page_number_confidence

80

Page_number_module_version

1.0.5

Page_range

1-15

Pages

15

Pdf_degraded

invalid-jp2-headers

Pdf_module_version

0.0.25

Possible copyright status

In copyright. Digitized with the permission of the rights holder.

Ppi

300

Source

Biodiversity Data Journal 11

Year

2023

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